Why this pipeline.
Why DidemBio exists, why it's built the way it is, and the lines it won't cross.
A talented student loses months to a framework that keeps shifting under them; a PI hands the data to a service and waits on a deck that doesn't answer the question they asked. The reason isn't that the analysis is hard; it's that the existing tooling assumes you have a bioinformatics team.
Most labs don't. They have a question, a dataset, and a deadline. So we built the analysis layer a lab would build for itself, if it had the time: one workflow per modality, the same shape across all of them, documented analysis settings, and a Methods paragraph you can review and adapt for your manuscript.
It is opinionated by design. There are not fifty parameters to tune. The analyses we ship are the ones we are willing to defend in peer review, and the data your lab actually generates, transcriptomic or cytometric, runs through one layer instead of two ecosystems and a separate vendor. If your data needs something the pipeline does not do, you ask. We answer.